Abstract: Objective To describe the methods of single-incision laparoscopic appendectomy and our experience with it. Methods Fifty patients with appendicitis admitted to our hospital from October 2009 to March 2010 were enrolled in this study.The patients underwent single-incision laparoscopic appendectomy with 3 trocars inserted around the umbilicus(one was 12mm long and two were 5mm long).The incision was called a single incision when its length was >20mm.The appendix was pulled out from the 12mm-long trocar.If the appendix was large,two 5mm-long trocars or one 5mm-long trocar was fused into a single incision to form a new single incision to remove the appendix.Then,the outcomes of single-incision laparoscopic appendectomy and traditional triple-hole laparoscopic appendectomy were compared. Results The average operation time of single-incision laparoscopic appendectomy was 15min(range 10-20min).The hospital stay time of the patients was 2-3days after operation.No obvious abdominal scar formation,incision infection,appendix stump inflammation and adhesion occurred after operation. Conclusion Single-incision laparoscopic appendectomy is a simple and safe procedure for appendicitis with extremely small trauma.