Abstract: Objective To observe the changes of drug-sensitivity in wounds before and after infection with dominant bacteria in seawater. Methods Fifty-six specific pathogen-free（SPF） mice after injury were randomly divided into experimental group and negative control group.Mice in experimental group were immersed in artificial seawater containing marine bacteria and those in negative control group were immersed in seawater not containing marine bacteria,and then fed in aseptic containers.Secretion from wounds of experimental mice was cultured and identified with their drug sensitivity tested.Drug sensitivity of the bacteria before and after immersion was compared. Results The average resistance of the bacteria isolated from wound in mice to ampicillin and piperacilin was 62% and 15.4%,respectively,before and after immersion.Their sensitivity to gentamicin,SMZco,cefotaxime,imipenem,levofloxacin,norfloxacin,chloramphenicol,and cefotaxime/clav was the highest and these antibiotics should be recommended as the first choice for the wounds infected with marine bacteria. Conclusion Vibrio in a coastal area is highly resistant to ampicillin and its drug sensitivity spectra will change in wounds before and after infection with dominant bacteria.