Abstract: Objective To study the relation between nursing stress and job satisfaction in nurses with different professional titles. Methods Nursing stress resources and job satisfaction in 1 000 nurses were investigated with nurse stressor scale and satisfaction scale.Their relation was analyzed. Results The nursing stress was the highest in assistant nursing technician,followed by nursing technician,nurses,associate chief nurses and above.The job satisfaction score was the highest in associate chief nursing technician,nursing technician,nurses and assistant nursing technician.The nursing stress was negatively related with the job satisfaction in nurse with different professional titles（P＜0.01）. Conclusion The nursing stress is closely related with the job satisfaction in nurse with different professional titles.Effective measures should thus be taken to reduce their nursing stresses and improve their job satisfaction.