串珠素小干扰RNA对结直肠癌细胞增殖的影响

刘洪金; 赵忠鹏; 付艳; 杨鹏辉; 段跃强; 门丽; 赵晖; 杜楠; 王希良

doi:10.3969/j.issn.2095-5227.2014.06.028

串珠素小干扰RNA对结直肠癌细胞增殖的影响

Effect of perlecan siRNA on proliferation of colorectal carcinoma cells

摘要

摘要: 目的评估串珠素小干扰RNA （siRNA）对肿瘤细胞增殖的影响。方法体外培养HCT15和HT29结直肠癌细胞，将构建好的串珠素siRNA载体转染培养细胞中，荧光显微镜下观察转染效率，qRT-PCR法检测串珠素mRNA表达，Western blot法检测串珠素蛋白表达，MTS法检测串珠素siRNA对肿瘤细胞增殖的影响。结果串珠素siRNA转染肿瘤细胞48 h后，肿瘤细胞串珠素mRNA、蛋白水平均出现降低（P< 0.05）。串珠素siRNA对肿瘤细胞增殖有明显的抑制作用（P< 0.01）。结论串珠素siRNA对结直肠癌肿瘤细胞增殖具有显著的抑制效果。

Abstract: Objective To assess the effect of perlecan siRNA on proliferation of colorectal carcinoma cells. Methods Colorectal carcinoma HCT15 and HT29 cells were cultured in vitro. The constructed perlecan siRNA vector was transfected into HCT15 and HT29 cells. The transfection rate was observed under a fl uorescence microscope. Expression of perlecan siRNA and protein was detected by RT-PCR and Western blot, respectively. Effect of perlecan siRNA on proliferation of HCT15 and HT29 cells was detected by MTS. Results The expression levels of perlecan mRNA and protein were significantly lower in HCT15 and HT29 cells 48 h after transfected with perlecan siRNA (P< 0.05). Perlecan siRNA significantly inhibited the proliferation of HCT15 and HT29 cells (P< 0.01). Conclusion Perlecan siRNA can significantly inhibit the proliferation of colorectal carcinoma cells.

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