Abstract: Objective To investigate the features of the fatigue caused by high intensive short-term military operations and regulation changes of the nerves- endocrine- system. Methods Clinical data about 240 of ficers who took high intensive short-term military operations for seven days, including the pituitary – adrenal hormone (ACTH, Cortisol, UFC), pituitary – gonadal hormone (LH, FSH, T, E2), pituitary – thyroid hormone (TSH, TT3, T3, TT4, T4), were detected and analyzed using Fatigue Assessment Scale. Results After the 7-day military operations, the subjects' feeling of unpleasurement in faigue scales signi ficantly increased (1.47±0.44 vs 1.61±0.52, P＜ 0.05), while the adrenal cortex function reduced (Cortisol from 336.97±105.23 nmol/L to 283.94±84.61 nmol/L, UFC from 426.39±162.35 nmol/24 h to 371.76±155.51 nmol/24 h), testosterone level reduced (T from 23.51±6.49 nmol/L to 18.89±5.89 nmol/L), and thyroid function increased (TT3 from 2.13±0.30 nmol/L to 2.72±2.26 nmol/L, FT4 from 13.45±1.45 pmol/L to 15.92±1.95 pmol/L, FT3 from 5.23±0.42 pmol/L to 5.61±0.58 pmol/L). Conclusion High intensive short-term operations can increase the feeling of fatigue and then seriously affect the psychology Neuro - endocrine system of the of ficers.