Abstract: Objective To analyze the association between dominant symptoms in different subtypes of functional dyspepsia (FD) and Helicobacter pylori (Hp) infection. Methods Patients matched with FD criterion of RomeⅢwere enrolled in the outpatient service. After investigating the dominant symptoms, they were divided into three subtypes: postprandial distress syndrome (PDS), epigastric pain syndrome (EPS) and overlapped (OL) group. Main symptoms and degree of their severity in patients were evaluated by 5-H gastric emptying test and drinking load test. The differences between Hp infection, gastric emptying and visceral perceptive threshold of different subtypes of patients, and the association between single symptom and pathophysiologic changes mentioned above were analyzed. Results There were 108 patients enrolled in this study. The Hp infection rate in EPS (35.7%, 10/28) was higher than that in PDS (16%, 8/50, P=0.038) and OL (10%, 3/30, P=0.021). Patients with abdominal distension showed low Hp infection rate. The PDS patients showed slowly gastric emptying, but patients suffered with early satiety showed rapid gastric emptying. The maximum tolerance value of patients suffered from postprandial fullness and belching decreased. Conclusion Different subtypes of FD patients have different Hp infection rate, while the Hp infection is not associated with symptoms and pathophysiological changes. Gastric motility and sensation changes are closely related to some special symptoms, and dominant symptoms of patients should be paid attention to during treatment.