Abstract: Objective To evaluate the ectopic pregnancy (EP) rate of embryo transfer in assisted reproductive technologies (ART) using transabdominal longitudinal section scanning or modified method of ultrasound-guidance. Methods The EP rate of patients undergoing embryo transfer by two different methods of ultrasound-guidance from January 2014 to September 2015 in our ART center was retrospectively analyzed.The traditional method ( group A) was simply scanned the endometrium lining longitudinally by putting the tip of the catheter 0.5-1 cm beyond fundus, and the modified method ( group B) was to put the tip by longitudinal section at first, then adjust the catheter depth by transverse section scanning. Results There were 333 cycles in group A with average age of (33.81±4.77) years and 295 cycles in group B with average age of (34.29±4.71) years.There were no significant differences in mean age and clinical pregnancy (CP) rate between two groups.While, the EP rate in group B was significantly lower than group A (0.3% vs 2.4%, P=0.030). Conclusion This study suggests that implanting the embryos under theguidance of modified method of longitudinal combined transverse section scanning can reduce the EP rate in ART, which is an effective and safe technique for embryo transfer.