Abstract: Objective To explore the relationship between serum 25-hydroxy vitamin D and lipid profiles in patients with type 2 diabetes mellitus (T2DM). Methods This cohort included 408 patients diagnosed as T2DM from a large epidemiological survey database.After medical history taking and physical examination, every patient underwent serum biochemical tests and then were divided into three groups: normal 25(OH)D＞ 50 nmol/L, insuf ficient 25≤25(OH)D≤50 nmol/L, de ficient 25(OH)D levels＜ 25 nmol/L according to the serum 25(OH)D level.AVONA was used to analyze and compare the lipid levels in different 25(OH)D groups, and multivariate logistic regression was used to analyze the relationship between TC, LDL and 25(OH)D. Results Both TC and LDL-C in de ficient group were signi ficant higher than those in normal and insuf ficient group TC: (5.59±1.0) mmol/L vs (5.26±0.96) mmol/L, (5.25±1.07) mmol/L, P＜ 0.05, respectively; LDL-C: (3.71±0.71) mmol/L vs (3.41±0.76) mmol/L, (3.45±0.84) mmol/L, P＜ 0.05, respectively, while no signi ficant difference was found in TG and HDL-C (P_TC=0.405, P_LDL-C=0.719).After adjusting age, FPG and BMI, the serum 25-hydroxy vitamin D was negatively correlated with TC and LDL-C respectively. Conclusion The TC and LDL-C are signi ficantly different in different level of serum 25-hydroxy vitamin D groups.The serum 25-hydroxy vitamin D is negatively associated with TC and LDL-C in T2DM patients.