Abstract: Objective To observe the effect of aspartame and its compositions on postprandial glucose metabolism in Kunming and KKAy mice. Methods Kunming and KKAy mice were randomly divided into intervention group and control group,12 in each group.Mice in intervention group received intra-gastric aspartame or its composition(aspartic acid or phenylalanine at the dose of 2mg/（g·kg） plus glucose or starch,while those in control group received only glucose or starch at the dose of 2mg/（g·kg）.Blood samples were collected from vena caudalis of the mice and blood glucose level was measured in mice 0.5,1 and 2h after intra-gastric intervention with drugs.Blood glucose level and AUC at different intervention time points were compared between two groups. Results Glucose tolerance test and starch tolerance test showed that the blood glucose level and AUC at different intervention time points were significantly lower in intervention group than in control group. Conclusion Aspartame and its compositions（aspartic acid and phenylalanine） can decrease the postprandial glucose level and AUC in Kunming or KKAy mice.