DUAN Fei, YANG Lin, LIU Hongqi, YANG Yanmei, HU Yawen, GU Bin. Effect of cold atmospheric plasma on healing of oral and maxillofacial wound of rats[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2024, 45(6): 652-658, 688. DOI: 10.12435/j.issn.2095-5227.2024.064
Citation: DUAN Fei, YANG Lin, LIU Hongqi, YANG Yanmei, HU Yawen, GU Bin. Effect of cold atmospheric plasma on healing of oral and maxillofacial wound of rats[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2024, 45(6): 652-658, 688. DOI: 10.12435/j.issn.2095-5227.2024.064

Effect of cold atmospheric plasma on healing of oral and maxillofacial wound of rats

More Information
  • Corresponding author:

    GU Bin. Email: gubinmail301@163.com

  • Received Date: February 21, 2024
  • Available Online: June 10, 2024
  • Background 

    Cold atmospheric plasma (CAP) has gradually drawn attention in the medical field due to its sterilization, promotion of cell proliferation, angiogenesis, and collagen synthesis. However, the application of CAP in the treatment of oral and maxillofacial wounds still needs to be explored.

    Objective 

    To investigate the effect of CAP on the healing of oral and maxillofacial wounds in rats and provide experimental evidence for the application of CAP in the treatment of oral and maxillofacial wounds.

    Methods 

    CCK-8 assay and cell migration assay were used to evaluate the effects of CAP on the proliferation and migration of rat oral mucosal fibroblasts under different parameters to determine the optimal experimental parameters. Under inflammatory induction conditions, real-time quantitative PCR and Western blot were used to detect the levels of inflammatory-related indicators (TNF-α, IL-1β, IL-6) and repair-related indicators (TGF-β, VEGF, COL1A1) of rat oral mucosal fibroblasts after CAP treatment. A total of 36 SPF male SD rats were selected to establish a cheek penetrating wound model. The experimental animals were randomly divided into control group (n=18) and CAP group (n=18). The wounds in the control group were allowed to heal naturally, while the wounds in the CAP group were treated with CAP daily. The changes in the oral and maxillofacial wound tissues of rats were observed by HE staining, Masson staining and IHC staining. Western blot was used to compare the protein expression of inflammatory-related indicators (TNF-α, IL-1β, IL-6) and repair-related indicators (TGF-β, VEGF, COL1A1) in the two groups.

    Results 

    CAP treatment could improve the proliferation and migration ability of rat oral mucosal fibroblasts (P<0.05), and 40%-30S was the optimal experimental equipment parameter. Under this parameter, CAP could significantly down-regulate the expression of inflammatory-related indicators (TNF-α, IL-1β, IL-6) of rat oral mucosal fibroblasts under the inflammatory microenvironment (P<0.05), while significantly up-regulate the expression of repair-related indicators (TGF-β, VEGF, COL1A1). Animal experiments further confirmed that the degree of wound healing in the CAP group was superior to that of the control group. The results of HE and Masson staining showed that the skin and oral mucosa growth at the edge of the wound in the CAP group were more coherent, and collagen fiber proliferation was more obvious. IHC staining showed that the repair-related indicators COL1A1 and VEGF were expressed higher in the skin and oral mucosal tissues of the wound in the CAP group. Western blot results showed that compared with the control group, the expression of inflammatory indicators (TNF-α, IL-1β, IL-6) in the wound tissues of the CAP group animals was relatively lower (P<0.05), while the expression levels of repair-related indicators VEGF and COL1A1 were higher (P<0.05), which was consistent with the results of animal slice observation.

    Conclusion 

    CAP treatment can promote the rapid healing of oral and maxillofacial wounds by promoting cell proliferation and migration, alleviating inflammatory reactions, and enhancing the expression of repair-related factors, reducing the risk of delayed wound

  • [1]
    Moszczyńska J,Roszek K,Wiśniewski M. Non-thermal plasma application in medicine-focus on reactive species involvement[J]. Int J Mol Sci,2023,24(16): 12667. doi: 10.3390/ijms241612667
    [2]
    Sakudo A,Yagyu Y,Onodera T. Disinfection and sterilization using plasma technology:fundamentals and future perspectives for biological applications[J]. Int J Mol Sci,2019,20(20): 5216. doi: 10.3390/ijms20205216
    [3]
    李明,刘建恒,崔翔,等. 等离子体处理技术促进高分子聚合物在骨组织工程中的应用综述[J]. 解放军医学院学报,2020,41(1): 84-87.
    [4]
    岑瑞祥,万浪,刘昀,等. 低温等离子刀扁桃体切除术疗效的Meta分析[J]. 解放军医学院学报,2015,36(2): 144-147.
    [5]
    谢娜,刘飞,李子夏,等. 大气压低温等离子体在口腔医学中应用进展[J]. 中国医学物理学杂志,2021,38(2): 245-249.
    [6]
    Gümbel D,Bekeschus S,Gelbrich N,et al. Cold atmospheric plasma in the treatment of osteosarcoma[J]. Int J Mol Sci,2017,18(9): 2004. doi: 10.3390/ijms18092004
    [7]
    Zhai SY,Kong MG,Xia YM. Cold atmospheric plasma amelio-rates skin diseases involving reactive oxygen/nitrogen species-mediated functions[J]. Front Immunol,2022,13: 868386. doi: 10.3389/fimmu.2022.868386
    [8]
    Limanowski R,Yan DY,Li L,et al. Preclinical cold atmospheric plasma cancer treatment[J]. Cancers,2022,14(14): 3461. doi: 10.3390/cancers14143461
    [9]
    Gan L,Jiang J,Duan JW,et al. Cold atmospheric plasma applications in dermatology:a systematic review[J]. J Biophotonics,2021,14(3): e202000415. doi: 10.1002/jbio.202000415
    [10]
    Brand HS,Ligtenberg AJM,Veerman ECI. Saliva and wound healing[J]. Monogr Oral Sci,2014,24: 52-60.
    [11]
    Khoury ZH,Brooks JK,Bashirelahi N. What every dentist should know about oral mucosal wound healing[J]. Gen Dent,2020,68(6): 24-26.
    [12]
    Hao M,Wang DX,Duan MN,et al. Functional drug-delivery hydrogels for oral and maxillofacial wound healing[J]. Front Bioeng Biotechnol,2023,11: 1241660. doi: 10.3389/fbioe.2023.1241660
    [13]
    Oren D,Dror AA,Zoabi A,et al. The impact of delayed surgical intervention following high velocity maxillofacial injuries[J]. Sci Rep,2021,11(1): 1379. doi: 10.1038/s41598-021-80973-7
    [14]
    Almadani YH,Vorstenbosch J,Davison PG,et al. Wound healing:a comprehensive review[J]. Semin Plast Surg,2021,35(3): 141-144. doi: 10.1055/s-0041-1731791
    [15]
    Pan YL,Zhu H,Hou LL. Epidemiological analysis and emergency nursing care of oral and craniomaxillofacial trauma:a narrative review[J]. Ann Palliat Med,2022,11(4): 1518-1525. doi: 10.21037/apm-21-2995
    [16]
    Wusiman P,Maimaitituerxun B,Guli,et al. Epidemiology and pattern of oral and maxillofacial trauma[J]. J Craniofac Surg,2020,31(5): e517-e520. doi: 10.1097/SCS.0000000000006719
    [17]
    Yan X,Ouyang JT,Zhang CY,et al. Plasma medicine for neuroscience-an introduction[J]. Chin Neurosurg J,2019,5: 25. doi: 10.1186/s41016-019-0172-9
    [18]
    Von Woedtke T,Schmidt A,Bekeschus S,et al. Plasma Medicine:A Field of Applied Redox Biology[J/OL]. https://doi.org/10.21873/invivo.11570.
    [19]
    Anitua E,Troya M,Zalduendo M,et al. Personalized plasma-based medicine to treat age-related diseases[J]. Mater Sci Eng C Mater Biol Appl,2017,74: 459-464. doi: 10.1016/j.msec.2016.12.040
    [20]
    Verdolino DV,Thomason HA,Fotticchia A,et al. Wound dressings:curbing inflammation in chronic wound healing[J]. Emerg Top Life Sci,2021,5(4): 523-537. doi: 10.1042/ETLS20200346
    [21]
    Li MR,Hou Q,Zhong LZ,et al. Macrophage related chronic inflammation in non-healing wounds[J]. Front Immunol,2021,12: 681710. doi: 10.3389/fimmu.2021.681710
    [22]
    Moretti L,Stalfort J,Barker TH,et al. The interplay of fibroblasts,the extracellular matrix,and inflammation in scar formation[J]. J Biol Chem,2022,298(2): 101530. doi: 10.1016/j.jbc.2021.101530
    [23]
    Arndt S,Landthaler M,Zimmermann JL,et al. Effects of cold atmospheric plasma (CAP) on ß-defensins,inflammatory cytokines,and apoptosis-related molecules in keratinocytes in vitro and in vivo[J]. PLoS One,2015,10(3): e0120041. doi: 10.1371/journal.pone.0120041
    [24]
    Hassanshahi A,Moradzad M,Ghalamkari S,et al. Macrophage-mediated inflammation in skin wound healing[J]. Cells,2022,11(19): 2953. doi: 10.3390/cells11192953
    [25]
    Guo S,Dipietro LA. Factors affecting wound healing[J]. J Dent Res,2010,89(3): 219-229. doi: 10.1177/0022034509359125
    [26]
    Rezaie A,Leite GGS,Melmed GY,et al. Ultraviolet A light effectively reduces bacteria and viruses including coronavirus[J]. PLoS One,2020,15(7): e0236199. doi: 10.1371/journal.pone.0236199
    [27]
    Bainbridge P. Wound healing and the role of fibroblasts[J]. J Wound Care,2013,22(8): 407-408,410-412. doi: 10.12968/jowc.2013.22.8.407
    [28]
    desJardins-Park HE,Foster DS,Longaker MT. Fibroblasts and wound healing:an update[J]. Regen Med,2018,13(5): 491-495. doi: 10.2217/rme-2018-0073
    [29]
    Talbott HE,Mascharak S,Griffin M,et al. Wound healing,fibroblast heterogeneity,and fibrosis[J]. Cell Stem Cell,2022,29(8): 1161-1180. doi: 10.1016/j.stem.2022.07.006
    [30]
    Wang XJ,Han GW,Owens P,et al. Role of TGF beta-mediated inflammation in cutaneous wound healing[J]. J Investig Dermatol Symp Proc,2006,11(1): 112-117. doi: 10.1038/sj.jidsymp.5650004
    [31]
    Lichtman MK,Otero-Vinas M,Falanga V. Transforming growth factor beta (TGF-β) isoforms in wound healing and fibrosis[J]. and,2016,24(2): 215-222.
    [32]
    Arndt S,Unger P,Berneburg M,et al. Cold atmospheric plasma (CAP) activates angiogenesis-related molecules in skin keratinocytes,fibroblasts and endothelial cells and improves wound angiogenesis in an autocrine and paracrine mode[J]. J Dermatol Sci,2018,89(2): 181-190. doi: 10.1016/j.jdermsci.2017.11.008
    [33]
    Xiaojie XJ,Banda J,Qi H,et al. Scarless wound healing:current insights from the perspectives of TGF-β,KGF-1,and KGF-2[J]. Cytokine Growth Factor Rev,2022,66: 26-37. doi: 10.1016/j.cytogfr.2022.03.001
    [34]
    Bekeschus S,von Woedtke T,Emmert S,et al. Medical gas plasma-stimulated wound healing:evidence and mechanisms[J]. Redox Biol,2021,46: 102116. doi: 10.1016/j.redox.2021.102116
    [35]
    Guo L,Zhao YM,Liu DX,et al. Cold atmospheric-pressure plasma induces DNA-protein crosslinks through protein oxidation[J]. Free Radic Res,2018,52(7): 783-798. doi: 10.1080/10715762.2018.1471476
    [36]
    Kim N,Lee S,Lee S,et al. Portable cold atmospheric plasma patch-mediated skin anti-inflammatory therapy[J]. Adv Sci (Weinh),2022,9(34): e2202800. doi: 10.1002/advs.202202800

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