Screening and identification of genes transactivated by the Pdomain protein of hepatitis Bvirus DNA polymerase using cDNA microarray

Objective:To study the difference in gene expression in human hepatoblastoma cell line HepG2 cells transfected with PR of hepatitis B virus DNA polymerase expressing plasmid by cDNA microarray assay and further elucidate its molecular biological function.Methods:Sequence specific primers were designed and synthesized and the PR coding DNA fragment was amplified with polymerase chain reaction （PCR） technique. The expressive vector of pcDNA3.1（-）-PR was constructed by routine molecular biological methods. cDNA microarray technology was employed to detect the mRNA from the HepG2 cells transfected with pcDNA3.1（-）-PR and pcDNA3.1（-）, respectively using lipofectamine. Results:The scanning results indicate that among 1 152 genes which were gotten from gene expression profile analysis, there were 79 genes were up-regulated and 90 genes were down-regulated in PR-expressing HepG2 cells.Conclusion:cDNA microarray technology was successfully used to screen the genes differentially expressed in PR-expressing HepG2 cells, which brought some new clues for studying the potential molecular mechanism of PR of protein.