The effects on glucose levels in Kkay and Kk mice by high-caloric diet

Objective:To observe the effects of high-caloric diet on type 2 diabetes in Kkay and Kk mice. Methods:Kkay, Kk and C57BL/6 mice were randomly distributed into high-caloric （A） or routine diet （B） group, and the glucose levels were tested in the week 0 and week 4 of experiment. Oral glucose tolerance test （OGTT） was performed in mice with normal glucose after high-caloric diet. Another group mice on high-caloric diet for 4 weeks were fed on food B for 8 weeks to observe the change of glucose. In the end of each experiment the pathological changes of islets were observed. Results:Compared with food B group, food A group of Kkay and Kk mice had higher glucose level, prevalence of diabetes and weight gain, while C57 mice of food A group got weight gain to some certain but no increase of glucose level. OGTT showed that there was a higher glucose curve peak in Kkay mice while a delayed higher glucose curve peak in Kk mice. And another 3/8 （37.5%） diabetic Kkay mice and 5/7 （71.43%） Kk mice with normal routine glucose were diagnosed by OGTT. After 8 weeks of routine food to food A group, glucose levels of Kkay and Kk mice decreased obviously compared with C57 mice although all of the three kinds mice had a weight loss. Pathological analysis showed that the pancreatic islet size of Kkay and Kk mice on high energy were very large due to hyperplasia and hypertrophy of B-cells, which was partly relieved after dietary restriction.Conclusions:High-caloric diet could increase the glucose level,the prevalence of diabetes and pancreatic islet size of Kkay and Kk mice, which can be relieved by dietary restriction; Kkay and Kk mice had abnormal response to OGTT, especially the latter.