Prognosis of endothelial chimerism in renal allograft

Objective: To investigate the presence of endothelial chimerism and its relationship with prognosis of renal allograft.Methods: We studied biopsy samples from 34 renal transplants of female recipients who received the kidney from a male donor for the presence of endothelial cells of recipient origin.Formalin-fixed,paraffin-embedded tissue sections of renal biopsy samples were examined by fluorescence in situ hybridization for the presence of endothelial cells containing two X-chromosomes using a biotinylated Y-chromosome probe and digoxigenin labeled X-chromosome probe.Results: The FISH methods did identify endothelial cells of recipient origin.The difference of serum creatinine post one year of transplantation in the group of presence or absence of endothelial chimerism was not statistically significant（115.88±25.06）mol/L vs（118.16±18.85）mol/L（P＞0.05）.Conclusion: The impact of endothelial chimerism on renal allograft long time survival needs continue follow-up visits.