Design of 60 mer oligonucleotide probes for microarray detection and genotype of hepatitis virus

Objective: To design oligonucleotide probes for microarray detection and genotype of hepatitis virus.Methods: By analyzing the DNA or cDNA of HBV,HDV and HCV of 6 different genotypes with BLAST program,a group of specific sequences for the candidate probes was specified.Array designer 3.0 software was applied to analyze the candidates to select the probes with high specificity,identical length and similar melting temperature（Tm）.Results: Altogether 156 oligonucleotide probes were designed,and deposited on oligonucleotide chips as the microarray for hepatitis virus detection and genotype.Conclusion: BLAST and Array designer 3.0 software are effective for designing the oligonucleotide probes.