Expression and purification of prostate stem cell antigen and heat shock protein 70 carboxyl-terminus

Objective To lay the foundation for the development of tumor vaccine by constructing,expressing and purifying recombinant plasmid of prostate stem cell antigen（PSCA） and heat shock protein 70 carboxyl-terminus（HSPC70）. Methods Recombinant plasmid pET21-PSCA-HSPC was constructed by cloning PSCA gene and carboxyl-terminus gene of HSP70.Expression of recombinant plasmid pET21-PSCA-HSPC was induced with IPTG and identified by Western blot analysis and then recombinant plasmid pET21-PSCA-HSPC was purified. Results The recombinant plasmid pET21-PSCA-HSPC was successfully constructed.SDS-PAGE showed PSCA-HSPC in a soluble form.The purity of recombinant protein PSCA-HSPC was over 95% after purification with Ni-ATA,phenyl sephrose FF and Superdex 75. Conclusion Recombinant fusion protein PSCA-HSPC is expressed in soluble form.