Proteomics of space serratia marcescens LCT-SM166 strain

Objective To analyze and identify the proteins with different expressions in serratia marcescens loaded in Shenzhou 8 spacecraft. Methods The serratia marcescens strain LCT-SM166 loaded in Shenzhou 8 spacecraft and ground control strain LCTSM213 were isolated and their 16sRN was identified by isobaric tags for relative and absolute quantitation (iTRAQ). The proteins of serratia marcescens LCT-SM166 and LCT-SM213 strains were detected by mass spectrometry and their differential expression was analyzed. Results Among the 111 differentially expressed proteins in the 1 713 possible proteins identified in this study, the expression was up-regulated in 20 and down-regulated in 91. Gene ontology (GO) enrichment analysis showed that the majority of differentially expressed proteins were related to bacterial metabolism. Conclusion Space environment can affect the protein expressions of serratia marcescens. The differentially expressed proteins mainly participate in the bacterial metabolic process.Proteomic analysis of space serratia marcescens mutants can lay a foundation for further transomics analysis.