Expression of metadherin gene in metastatic breast cancer and its clinical significance

Objective To study the expression of metadherin(MTDH) in patients with metastatic breast cancer. Methods Expressions of human breast cancer cell lines MCF-7, SK-BR3 and MDA-MB-231 of MTDH gene were detected with cellular immunochemical method.Expression of MTDH gene was detected in paraffin samples from 60 metastatic breast cancer patients with immunohistochemical method. Results The MTDH gene was mainly expressed in cell membrane or cytoplasm of human breast cancer cell lines.The positive MTDH gene expression rate was significantly higher in metastatic breast cancer patients than in primary breast cancer patients(73.8% vs 38.9%, P< 0.05).The expression of MTDH gene was significantly related with the clinical stage, metastatic site, progress time, Ki67, P53 and histological type of breast cancer(P< 0.05), but not related with the age, primary lymph node metastasis, HER-2, ER, and PR(P> 0.05). Conclusion MTDH gene can be expected to become an independent factor for the prognosis of breast cancer patients