Effect of platelet rich fibrin on proliferation and differentiation of human gingival fibroblasts on rough titanium surface

Objective To study the effect of platelet rich fibrin (PRF) on proliferation and differentiation of human gingival fibroblasts on rough titanium surface. Methods Human gingival fibroblasts were cultured in vitro, and their fourth generation was co-cultured with rough titanium.The cells were then cultured in common medium and PRF medium respectively, and detected by MTT, RT-PCR and Western blot respectively. Results MTT showed that PRF could promote the proliferation of human gingival fibroblasts(P< 0.05).RT-PCR demonstrated that the expression level of type Ⅰcollagen mRNA was higher than that of bFGF mRNA in PRF medium than in common medium(P< 0.05).Western blot displayed that the expression level of typeⅠ collagen was higher in PRF medium than in common medium(P< 0.05). Conclusion PRF can accelerate the proliferation and differentiation of human gingival fibroblasts on rough titanium surface.