Lymphocyte cytosolic protein 1 regulates proliferation of diffuse large B-cell lymphoma cells

Objective To study the biological significance of lymphocyte cytosolic protein 1(LCP1) expression in diffuse large B-cell lymphoma(DLBCL) cells and its effect on proliferation of DLBCL cells. Methods Expression of LCP1 in healthy human B lymphoblastoid cells and various DLBCL cells was detected by Western blot.Over-expressed LCP1 was silenced in DLBCL cell line OCI-LY19.Vitality of the cells was tested with a CCK8 kit.Effect of LCP1 on cell cycle of OCI-LY19 cells was observed with PI staining. Results The expression level of LCP1 was significantly higher in DLBCL cells than in healthy human B lymphoblastoid cells.The vitality of OCI-LY19 cells decreased and the progressive cell sycle of OCI-LY19 cells was inhibited after LCP1 was silenced. Conclusion LCP1 is closely related with the proliferation of DLBCL cells and is an important biological marker for DLBCL.