Role of miR-217 in target regulation of differentiated NeuroD1 gene expression induced by insulin-secreting cells

Objective To identify the role of miR-217 in target regulation of NeuroD1 gene expression when bone marrow mesenchymal stem cells(BMSCs) are differentiated into insulin-secreting cells(IPCs). Methods BMSCs were isolated,cultured and differentiated into IPCs using rat pancreatic extraction(RPE).The relation between miR-217 and NeuroD1 target match was predicted using miRanda and TargetScan,and identified with the dual luciferase report system.Expression of miR-217 and NeuroD1 was detected by qRT-PCR when the BMSCs were differentiated into IPCs. Results The differentiated IPCs were stained scarlet with DTZ.Immunofluorescence cytochemistry showed the expression of insulin.The bioinformatics method showed that the miR-217 and NeuroD1 were well matched.Dual luciferase reporter system revealed that miR-217 was bound to 3'UTR of NeuroD1 mRNA and inhibited its expression.The qRT-PCR displayed that the expression of miR-217 was negatively correlated with that of NeuroD1 mRNA. Conclusion miR-217 can regulate the expression of NeuroD1 in the differentiation of IPC.