Effect of testosterone on secretion of anti-Mullerian hormone in mouse ovarian granular cells cultured in vitro

Objective To study the effect of testosterone on secretion of anti-Mullerian hormone(AMH) in mouse ovarian granular cells cultured in vitro. Methods Primary mouse ovarian granular cells were cultured in vitro and treated with testosterone at different concentrations. Expression of AMH in mouse ovarian granular cells was detected by ELISA. Results ELISA showed that the AMH was expressed in mouse ovarian granular cells. Twenty-four hours treatment of primary mouse ovarian granular cells with testosterone at the concentrations of 10^-8 mmol/L, 10^-7 mmol/L and 10^-6 mmol/L up-regulated their secretion of AMH in a concentration-and time-dependent manner(P<0.01). Conclusion Testosterone up-regulates the secretion of AMH in mouse ovarian granular cells in a concentration-dependent manner.