Serum ghrelin and somatostatin level in rats with experimental gastric ulcer under positive acceleration and its mechanism

Objective To study the serum ghrelin and somatostatin（SS） level in rats with experimental gastric ulcer under positive acceleration and its mechanism. Methods A rat chronic gastric ulcer model was induced by acetic acid.Twenty-four male SD rats with gastric ulcer were randomly divided into control group,+5 Gz group,and +10 Gz group（8 in each group）.The rats were treated 4 times by simulating positive acceleration every other day for 1 week 3 days after experiment.Gastric mucosa tissue and blood samples were taken on the next day after experiment.Pathological sections were stained with H＆E.Serum ghrelin and SS levels were measured by RIA. Results Optical microscopy showed abnormal morphology of disordered gastric mucosal glands with obvious infiltration of inflammatory cells when the +Gz was higher.The serum PGE2 level in gastric mucosa was 3.438±0.908 pg/ml in +10 Gz group,5.147±0.652 pg/ml in +5 Gz group and 6.986±0.743 pg/ml in control group（P＜0.05）.The serum ghrelin level was 94.48±23.96 ng/ml in +10 Gz group,142.56±38.63 ng/ml in +5 Gz group and 112.00±42.28 ng/ml in control group（P＜0.05）.The serum SS level was 32.65±11.68 pg/ml in +10 Gz group,51.52±10.88 pg/ml in +5 Gz group and 38.37±14.16 pg/ml in control group（P＜0.05）. Conclusion The ulcer healing is poor when the +Gz increases.The serum SS level increases when the +Gz maintains at a moderate level whereas the serum ghrelin level decreases when the +Gz maintains at a high level.