Reproduction and identification of LRP16 transgenic mice

Objective To establish the animal model for studying the in vivo biological function of LRP16 by reproducing and identifying LRP16 transgenic mice. Methods After the constructed LRP16 transgenic recombinant plasmid was transfected into 293T cells,whether it can effectively express the target protein was identified.The linear recombinant plasmid was injected into the fertilized ovum of FVB mice to produce F₀ mice.The positive F₀ mice were identified by PCR and reproduced. Results The constructed recombinant LRP16 plasmid was effectively expressed in 293T cells.Three positive F₀ mice were identified by PCR with a positive rate of 6.23%.One male F₀ mouse with the integrated LRP16 gene was hybridized with wild FVB mice to reproduce 7 F₁ mice,4 out of which were positive with a positive rate of 57.14%.One male F₁ mouse with the integrated LRP16 gene was hybridized with wild FVB mice to reproduce 5 F₂ mice,2 out of which were positive identified by PCR with a positive rate of 40%. Conclusion LRP16 gene is successfully integrated into the mouse genome and can maintain its inheritance,thus laying a foundation for further studying the LRP16 function in animals.