Effect of ASS on apoptosis and p-ERK1/2 expression in rats with myocardial ischemiareperfusion injury

Objective To study the effect of ASS on apoptosis and p-ERK1/2 expression in rats with myocardial ischemiareperfusion（I/R）injury. Methods Sixty SD rats were randomly divided into sham operation group（group C）,I/R group,ASS+Z group and ASS group.A rat myocardial I/R injury model was established by ligation of left anterior descending coronary artery.Myocardial infarction size was measured with nitroblue tetrazolium staining.Serum cTnT level was measured.Myocardial apoptotic index（AI） was assayed by TUNEL,and expressions of ERK1/2 and p-ERK1/2 were detected by Western blot. Results The serum cTnT level,myocardial infarction size and AI were significantly lower in ASS group than in IR group and ASS +Z group（P＜0.05）.The expression level of P-ERK1/2 was significantly higher in ASS group than in group C,IR group and ASS+Z group（P＜0.05）. Conclusion ASS can protect rats against I/R injury,attenuate myocardial apoptosis by activating the ERK1/2 signal pathway.