Effect of perlecan siRNA on proliferation of colorectal carcinoma cells

Objective To assess the effect of perlecan siRNA on proliferation of colorectal carcinoma cells. Methods Colorectal carcinoma HCT15 and HT29 cells were cultured in vitro. The constructed perlecan siRNA vector was transfected into HCT15 and HT29 cells. The transfection rate was observed under a fl uorescence microscope. Expression of perlecan siRNA and protein was detected by RT-PCR and Western blot, respectively. Effect of perlecan siRNA on proliferation of HCT15 and HT29 cells was detected by MTS. Results The expression levels of perlecan mRNA and protein were significantly lower in HCT15 and HT29 cells 48 h after transfected with perlecan siRNA (P< 0.05). Perlecan siRNA significantly inhibited the proliferation of HCT15 and HT29 cells (P< 0.01). Conclusion Perlecan siRNA can significantly inhibit the proliferation of colorectal carcinoma cells.