Expression and significance of long noncoding RNA in tibialis anterior muscle tissues of burned rats at early stage

Objective To explore the significance and changes of long noncoding RNA in metabolism of skeletal muscles at early stage in burned rats. Methods The rats were divided into experimental group (T) and control group (C). Within 72h after the injury, the tibialis anterior (TA) muscles were collected; TRIZOL method was applied for extraction of total RNA in tibialis anterior muscle, Arraystar company Rat LncRNA Array (4×44 k Arraystar) was used to detect the expression level of MRBC025014 and MRAK080917 in the tibialis anterior muscle of both experimental group and control group, real time quantitative PCR was used to verify the reliability of the microarray data. Results The chip screening results showed that compared with control group, the expression levels of MRAK080917 and MRBC025014 were significantly lower in experimental group (P=0.02, P=0.018). PCR data confirmed that the expression levels of MRAK080917 and MRBC025014 in experimental group were consistent with the results of chip screening (P＜ 0.05). Conclusion The expression levels of MRBC025014 and MRAK080917 down-regulate significantly in skeletal muscle at early stage after severe burn of rats, and its function in skeletal muscle metabolism may be related to regulating target genes.