Establishment and evaluation of loop-mediated isothermal amplification method for rapid detection of Helicobacter pylori

Objective To establish a Loop-mediated isothermal amplification method (LAMP) for detection of Helicobacter plyori through turbidity meter and colorimetric method and verify the specificity and sensitivity of this method. Methods Five sets of primers were designed to recognize the specific gene, UreB gene of H. pylori. The optimal set of primers was determined by amplification efficiency. Eighty gastric juice samples were collected to compare the sensitivity and specificity of LAMP with ¹³C urea breath test. Results The optimal primer for LAMP of H. pylori was HPUB3. All reactions were finished at a constant temperature in 60 minutes. The accuracy, sensitivity and specificity of LAMP in detection of H. pylori was 98.75%, 97.06% and 100%, which was not significantly different with those of ¹³C urea breath test (P > 0.05). Conclusion LAMP is a rapid method for detecting H. pylori with high sensitivity and specificity. This study lays a foundation for real-time, noninvasive, rapid detection of H. pylori during endoscopy examination.