MiR-1827 inhibits migration of lung adenocarcinoma A549 cell line and regulates c-Myc expression

Objective To investigate the effect of miR-1827 on the migration of lung adenocarcinoma cell line A549 and whether it regulates the expression of proto-oncogene c-Myc. Methods The bioinformatics method was used to predict the paired sites between miR-1827 and c-Myc gene mRNA 3' untranslated region(UTR). The miR-1827 mimics were transfected into the lung cancer cell line A549 using liposomes. Real-time PCR was performed to detect the expression of miR-1827 and c-Myc mRNA. The change of c-Myc protein expression was detected by western blot. Cell scratch assay and Transwell cell migration assay were performed to examine the effect of miR-1827 on the migration function of A549. A549 cells transfected with the miR-NC sequence were used as control. The dual luciferase reporter gene assay was used to identify whether miR-1827 directly bounded to c-Myc mRNA 3'UTR and specific binding sites. The association between miR-1827 expression and the prognosis of lung cancer patients was explored using published data from the GEO database. Results The online bioinformatics website Targetscan and DIANA-LAB showed that there was a mutual pairing sequence between the miR-1827 seed region and the c-Myc mRNA 3' UTR region. Compared with miR-NC transfected group, the expression of miR-1827 was significantly up-regulated, and the mRNA and protein levels of c-Myc gene decreased significantly in the miR-1827 transfected group. The dual luciferase reporter assay suggested that miR-1827 downregulated c-Myc expression by pairing with c-Myc mRNA 3'UTR directly. MiR-1827 could significantly inhibit the migration function of A549. The data from chip GSE63805 showed that the 8-year survival rate of the miR-1827 high expression group was higher than that of the miR-1827 low expression group. Conclusion MiR-1827 can regulate the expression of proto-oncogene c-Myc and inhibit the migration of A549 cell line.