Objective To explore the molecular characteristics in the metastatic progress of gastric cancer, and screen potential biomarkers for the precision diagnosis and target therapy of metastatic gastric cancer.
Methods The RNA-Seq data and DNA methylation data of gastric cancer patients were obtained from public datasets TCGA, and the differential genes between primary gastric cancer (n=158) and metastatic gastric cancer (n=176) were screened and the correlation analysis was carried out by R language.
Results A total of 170 differential genes were identified in the metastatic group, including 52 up-regulated and 118 down-regulated genes. Functional analysis revealed that those differential genes were mainly involved in immune regulation, cell differentiation, exogenous signal induction, autophagy and lysosomal transport, drug and protein metabolism and other signaling pathways. Further, risk analysis revealed that expressions of RTP3, GTF3C5, TMEM102, NHLRC3, FUT2, PGAP2 and AAMP, were related to the prognosis of gastric cancer.
Conclusion The combination of 7 genes identified in our study may become indicators for the prognostic evaluation of gastric cancer.
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