Background Chronic rhinosinusitis is a common chronic inflammatory disease. While the majority cases of this disease are not complicated with nasal polyps (chronic rhinosinusitis without nasal polyps, CRSsNP). However, the etiology and pathological mechanisms of CRSsNP remain unclear.
Objective To investigate the expression of sirtuin 1 (SIRT1) in epithelial cells of CRSsNP and determine its effects on TGF-β1-induced epithelial mesenchymal transition (EMT).
Methods Nasal epithelial cells (NECs) were collected from patients with CRSsNP and controls. SIRT1 expression was detected by means of Real-time PCR, immunohistochemistry and Western blot. In addition, human NECs were cultured under TGF-β1. The effects of SIRT1 on TGF-β1-induced EMT were investigated.
Results SIRT1was specifically down-regulated in patients with CRSsNP (P＜0.001). TGF-β1 decreased SIRT1 expression in vitro. Furthermore, the expression levels of E-cadherin were down-regulated, while the expression levels of α-SMA, vimentin and N-cadherin was up-regulated, and SIRT1-overexpression could reverse this process.
Conclusion SIRT1 suppress TGF-β1-induced EMT in human NECs, indicating that SIRT1 may play important roles in the pathogenesis of CRSsNP.
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