Construction of colon cancer organoids based on matrigel in vitro

  Background  Compared with traditional tumor models, the colon cancer organoids are closer to the structure and molecular characteristics of tumor tissues. The model can be applied to the research and development of new anti-colon cancer drugs and the exploration of tumor characteristics.

  Objective  To construct organoids of carcinoma of colon with Matrigel.

  Methods  HCT116 cells were stably cultured and resuspended. The cells were inoculated into the Matrigel, and then cultured in three-dimensional environment to form spherical (multi-leaf) colon cancer organoids with cavities. The development and morphological characteristics of colon cancer organoids were observed and analyzed by microscope. Different inoculation gradients (5×10³/mL, 5×10⁴/mL, 5×10⁵/mL) were set to observe the appropriate density of organoids development, HE staining was used to observe the colon cancer organoids morphology, and the effect of cisplatin (DDP) on colon cancer organoids was also observed.

  Results  Colon cancer cells were cultured in Matrigel for 6 days to form multi-cell globular structure, and some organoids developed lobular structure. The size of the organoids grew larger at density of 5×10⁴/mL or 5×10⁵/mL were better than those at density of 5×10³/mL. In addition, the research proved that cisplatin had obvious toxic effect on colon cancer organoids.

  Conclusion  In this experiment, culture conditions for human colon cancer organoids are successfully established, and the morphological characteristics of colon cancer organoids in vitro are observed, which provides a more simulated model for intensive research of colon cancer.