miR-143-3p inhibits invasion and migration of gastric cancer by targeting LASP1

XU Xinxin; LU Yixun; LI Kai; LIANG Wenquan; GAO Yunhe; XI Hongqing; WANG Xinxin; CHEN Lin

doi:10.3969/j.issn.2095-5227.2022.08.011

Volume 43 Issue 8

Sep. 2022

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ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL > 2022 > 43(8): 873-879, 886. > DOI: 10.3969/j.issn.2095-5227.2022.08.011

XU Xinxin, LU Yixun, LI Kai, LIANG Wenquan, GAO Yunhe, XI Hongqing, WANG Xinxin, CHEN Lin. miR-143-3p inhibits invasion and migration of gastric cancer by targeting LASP1[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2022, 43(8): 873-879, 886. DOI: 10.3969/j.issn.2095-5227.2022.08.011

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miR-143-3p inhibits invasion and migration of gastric cancer by targeting LASP1

XU Xinxin^{1, 2,},
LU Yixun^{1, 2},
LI Kai^{1, 2},
LIANG Wenquan²,
GAO Yunhe²,
XI Hongqing²,
WANG Xinxin²,
CHEN Lin^2, ,

1.
Chinese PLA Medical School, Beijing 100853, China
2.
Department of General Surgery, the First Medical Center, Chinese PLA General Hospital, Beijing 100853, China

Funds: Supported by the National Nature Science Foundation of China (81972790)

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Corresponding author:
CHEN Lin. Email: chenlinbj@sina.com
Received Date: April 14, 2022
Available Online: June 26, 2022

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Abstract

Abstract

Background Distant metastasis is a major risk factor affecting the prognosis of gastric cancer (GC) patients and often leads to treatment failure. Therefore, it is urgent to explore new therapeutic targets for tumor metastasis to improve the prognosis of GC patients.
Objective To explore whether the miRNAs we screened by bioinformatics analysis could inhibit the invasion and migration of GC cells by targeting LASP1.
Methods Based on TCGA database, the expression levels of miRNAs in GC and the correlation between LASP1 and miRNAs were analyzed by bioinformatic methods to predict miRNAs targeting LASP1. The targeted interaction between miR-143-3p and LASP1 was verified by dual-luciferase reporter assay. Using GC cell line MGC-803 as experimental object, miR-143-3p mimic, inhibitor and negative controls were transfected, respectively. Accordingly, the experiments were divided into miR-143-3p mimic negative control (NC) group, miR-143-3p mimic group, miR-143-3p inhibitor NC group and miR-143-3p inhibitor group. Transwell invasion assay and wound healing assay were applied to determine the changes of invasion and migration ability of MGC-803 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the messenger RNA (mRNA) expression level of LASP1 in MGC-803 cells after transfection, and western blot (WB) was used to detect the protein expression level of LASP1 in MGC-803 cells.
Results Bioinformatics analysis suggested that miR-143-3p might target LASP1 and the correlation between miR-143-3p and LASP1 was r=-0.284 (P＜0.01). The dual-luciferase reporter assay further confirmed that LASP1 was a direct target gene of miR-143-3p (P＜0.01). Transwell invasion assay and wound healing assay confirmed that miR-143-3p significantly inhibited the invasion and migration ability of gastric cancer MGC-803 cells. The results of qRT-PCR showed that the expression level of LASP1 in miR-143-3p mimic group was lower than that in miR-143-3p mimic NC group (P＜0.01), while the expression level of LASP1 in miR-143-3p inhibitor group had a higher expression level of LASP1 than that of the miR-143-3p inhibitor NC group (P＜0.05). The results of WB showed that protein changes of LASP1 were consistent with that of qRT-PCR.
Conclusion miR-143-3p could inhibit the invasion and migration of GC cells by targeting and down-regulating LASP1.
- gastric cancer,
- miR-143-3p,
- LIM and SH3 protein 1,
- cell invasion,
- cell migration

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miR-143-3p inhibits invasion and migration of gastric cancer by targeting LASP1

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