Background Xenogeneic cross circulation has some achievements in the field of organ preservation, but its effect on tissue preservation is still uncertain.
Objective To explore the effect of xenogeneic cross-circulation in preserving severed foot.
Methods The severed limb after amputation due to osteosarcoma was processed again and the limb was truncated approximately 5cm above the ankle join. Then, the main blood vessels of the broken foot without tumor were connected to the femoral artery and femoral vein of a Bama pig. The preservation effect was evaluated by tiptoe oxygen saturation, pulse rate, blood cell count and muscle histology.
Results Xenocross circulation was successfully performed to maintain the normal shape of the muscle tissue of the severed foot for more than 4 hours. Tiptoe oxygen saturation was 64% - 99%, the pulse rate was 45 - 106 bpm, the leukocytes count was (13.3 - 19.7) × 109/L, the lymphocytes count was (2.1 - 4.36) × 109/L, and the platelets count was (242 - 1 534) × 109/L. At 0 h and 4 h, the muscle fibers were normal. At 8 h, the muscle fibers were disordered and part of the tissue morphology was lost. At 0 h, the blood vessels of muscles were not filled. At 4 h, red blood cells passed through the blood vessels of muscles. At 8h, thrombus formed.
Conclusion Xenogeneic cross circulation can maintain the normal shape of muscle tissue of severed foot for more than 4h, while how to reduce the immune rejection still needs further research.
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