Background Lung cancer is one of the most common malignant tumors in the world, with the highest mortality. Compared with most tumors, the treatment of lung cancer is still not optimistic, which is a serious threat to human health. Protein ubiquitination is one of the post-translational modifications that regulate many cellular processes in different ways. Ubiquitin specific protease 39 (USP39) is closely related to the occurrence and development of a variety of tumors, but the mechanism in lung cancer remains to be explored.
Objective To construct a human lung cancer A549 cell line with stable and high expression of USP39, and observe the biological effects of high expression of USP39 on lung cancer A549 cells.
Methods Using lentivirus mediated USP39 overexpression vector, stable overexpression A549 USP39 cell line infected with lentivirus was screened by puromycin, and the results were verified by Western Blot analysis. Clone formation assay was used to detect the effect of USP39 gene overexpression on A549 cell proliferation. The effect of USP39 overexpression on the migration ability of A549 cells was detected by wound healing experiment.
Results Western blot showed that A549 USP39 overexpression stably transfected cell line was successfully constructed. Compared with the control group, the clone formation experiment showed that the stable and high expression of USP39 could significantly promote cell proliferation. Wound healing experiment showed that stable and high expression of USP39 could significantly increase the migration ability of A549 cells, and the difference was statistically significant (P < 0.05).
Conclusion The stable transfected A549 cell line with flag tag overexpression of USP39 is successfully constructed, and it is preliminarily verified that the overexpression of USP39 can significantly promote the proliferation and migration of tumor cells.
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