Two different methods for detection of calcitonin gene-related peptide in rats:A comparative study

Objective To evaluate the efficiency of radioimmunoassay（RIA） and enzyme linked immunoassay（ELISA） in detecting calcitonin gene-related peptide（CGRP） in rats. Methods CGRP levels in standard substance and quality control of CGRP,100 plasma samples from normal rats and middle cerebral artery occlusion（MCAO） model of rats were measured by RIA and ELISA,respectively,and their precision rates were compared. Results The relative coefficient of CGRP in normal rats and MCAO model of rats detected by RIA and ELISA was 0.986 and 0.962,respectively（P＜0.01）. Conclusion The CGRP detected by RIA is consistent with that detected by ELISA,thus both RIA and ELISA can be used in detection of CGRP.