Treatment of refractory non-Hodgkin’s lymphoma of the spleen with combined autologous cytokine induced killer cells and IL-2: A case report

Objective To observe the efficiency and safety of combined autologous cytokine induced killer（CIK） cells and IL-2 in treatment of splenic lymphoma in old patients. Methods Hepatic metastasis of splenic primary malignant lymphoma occurred in an old patient after splenectomy and 8 cycles of R-CHOP chemotherapy.Peripheral blood mononuclear cells（PBMC） were collected.CIK cells were induced with in vitro interferon gamma（IFN-γ）,IL-2 and anti-CD3 monoclonal antibody（mAb）.Liver function,tumor-related biological indexes and image changes were observed after 2-3×109 CIK cells were re-transfused into the patient each time and IL-2 100mU/d was subcutaneously injected for 10 days,28 days a cycle. Results No adverse reaction occurred in the patient after 8 cycles of CIK cells transfusion.The liver function and serum LDH level became normal（P＜0.05）.PET-CT showed that hepatic metastasis of lymphoma disappeared and completely relieved. Conclusion Combined autologous CIK cells and IL-2 is safe and effective for small residual or metastatic foci of lymphoma in old patients after splenectomy.