LIU Yan, LI Yang, LIN Kun, TIAN Miao, WANG Yu-tang, DAN Zhao-liang. Protective effect of piperine on H2O2-induced abnormal transient outward potassium current in rabbit atrial myocytes[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2012, 33(5): 496-499. DOI: CNKI:11-3275/R.20111212.1031.005
Citation: LIU Yan, LI Yang, LIN Kun, TIAN Miao, WANG Yu-tang, DAN Zhao-liang. Protective effect of piperine on H2O2-induced abnormal transient outward potassium current in rabbit atrial myocytes[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2012, 33(5): 496-499. DOI: CNKI:11-3275/R.20111212.1031.005

Protective effect of piperine on H2O2-induced abnormal transient outward potassium current in rabbit atrial myocytes

  • Objective To study the protective effect of piperine on H2O2-induced abnormal transient outward potassium current(Ito) in single rabbit atrial myocyte. Methods Effect of H2O2(50μmol/L) and piperine(7μmol/L) on Ito in single rabbit atrial myocyte was analyzed using the whole cell patch clamp technique. Results Piperine(7μmol/L) had no significant effect on Ito and its transient outward potassium channel dynamics.The peak value of Ito in rabbit atrial myocytes decreased to(32.8±2.0)pA/pF from(39.3±5.4)pA/pF(P<0.05),the current-voltage curve of Ito was shifted down,the channel steady-state activation curve of Ito was shifted to the right,the steady-state inactivation curve and the recovery time of Ito from inactivation did not change,the closed-state inactivation of Ito was accelerated,when H2O2(50μmol/L) was applied.Piperine(7μmol/L) significantly alleviated the inhibitory effect of H2O2 on Ito and its transient outward potassium channel dynamics(P<0.01). Conclusion Piperine can alleviate H2O2-induced abnormal Ito in rabbit atrial myocytes.
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