Specific suppression of GFP gene expression by siRNA of GFP in lung carcinoma cell A549

Objective: It was designed to investigate whether small interfering RNA （siRNA） of green fluorescence protein （GFP） can inhibit the expression of GFP in lung carcinoma cell A549. Methods: A plasmid included mU6 promoter and siRNA of GFP was designed and constructed. The plasmid was transfected into lung carcinoma cell A549. GFP-siRNA effect of interference to GFP is observed and evaluate in A549 cell. Results: GFP-RNAi can inhibit specific expression of GFP gene in lung carcinoma A549 cell line and mU6 plasmid, no-related-siRNA have no such specific effect. Conclusion: These observations may open a path forward the use of siRNA as a new tool for researching gene function in cancer cell line.