The construction and identification of cardic specific expression adenovirus vector

Objective: to establish a adenovirus vector that make foreign gene express in cardiomyocytes.Methods: we constructed a recombinant adenovirus （AdmlcGFP） in which contain the GFP gene was under the control of the ventricle specific myosin light chain-2 （mlc-2v）.Results: Our data demonstrated GFP was expressed in transfected neonate rat ventricular myocytes in vitro but not in transfected cell lines. Conclusion: the established viral vector AdmlcGFP allows heart specific expression of a foreign gene.