Cloning and finding of its new splicer of survivin gene from human gastric cancer

Objective：The purpose of this paper is to clone survivin（SVV）gene from human gastric cancer．Methods：The SVV cDNA was obtained by using RT-PCR method with total RNA extracted from the human gastric cancer cell line，SGC7901．After proved to be correct by sequencing ，recombinant expression plasmid p^RSET-SVV was transformed into E．coli BL21（DE3）．The fusion protein was produced by IPTG induction．Results：Obtained two SVV cDNA clones ，SVV-S₄A and SVV-S1B．The sequence of the previous is identical to that of the well-known SVV，but ，the third exon was missed in the latter．A new anticipated M_r 19500-protein band appeared on SDS-PAGE gel induced by IPTG．Conclusion：human SVV cDNA was cloned and a new splicer is found from human gastric cancer．SVV is highly expressedsioned in E．coli．