Retrovirus-mediated human IL-2 gene transfer in human bladder carcinoma cells

After retrovirus mediated human IL-2 gene expression vector pZip hIL-2 was packaged by PA 317 pcaking cell line, the retrovirus was obtained at a high titer （0.4-1.2×10 ⁵ cfu/ml）,and infected the human bladder cancer line EJ, On G418 resistance selection and hIL-2 activity assay, a clone （named EJ/hIL-2） secreting highest level of hIL-2（260 U/ml） was selected, and then Southern blot hybridization demonstrated that human IL-2 gene was integrated in the genome of EJ/hIL-2 cells. This laid the foundation to develop hIL-2 gene transducted tumor vaccine of human bladder cancer.