Increased ICAM-1 expression on epithelial cells induced by TNF-α

Objective:To study the effect of epithelial cell activation on the gene and cell surface expression of ICAM-1.Methods:BEAS-2B cells were activated by TNF-α（10 ng/ml） for 12h,ICAM-1 gene in BEAS-2B cells were analyzed by RT-PCR and cell surface ICAM-1 protein was assayed by Flow cytometry.Results:TNF-α upregulated ICAM-1 gene expression in BEAS-2B cells and increased ICAM-1 protein on BEAS-2B cell surface.Conclusion:TNF-α might induce BEAS-2B cells to express ICAM-1.