The relationship of the mutation on embB genes and drug-resistence with clinical treatment

Objective: To realize the relevance of the mutations of embB genes with drug resistence in M. tuberculosis isolates,and to evaluate their clinical value. Methods: 96 clinical isolates were identified their mycobacterial species,and then analyzed their embB genes with PCR SSCP. Results: 56 clinical isolates all had the same SSCP profiles as M.tuberculosis. 21 drug-sensitive isolates had normal embB SSCP profiles. Of 35 ethambutol-resistant isolates,17（48.6%） displayed abnormal embB SSCP profiles. Conclusions: Ethabutol resistancesin some M.tuberculosis isolates were due to mutations on embB genes,Moreover,the mutation of embB genes has high accidence in isolates which resistanthigh concentration ethambutol.