Morphology of SV40 transfection gene-immortalized liver cells in normal adults

Objective To study the effect of SV40 gene transfection on morphological structure of liver cells in normal adults and its special staining. Methods Liver cells （rSSR # 69/HL-7702 liver cells） containing SV40 gene from normal adults were stained with H＆E, glycogen and glucose-6-phosphatase （G-6-Pase）, and their morphological features and ultra-structure were observed under a light microscope and an electron microscope, respectively. Results No significant difference was found under light microscope in morphology of liver cells from normal adults before and after transfection. Glycogen staining showed glycogen pink granules in cytoplasm and G-6-Pase staining showed black iron particles in cytoplasm. Also, no significant difference was observed in ultrastructure of liver cells under electron microscope before and after transfection. Conclusion The morphological structure of liver cells from normal adults is not significantly different before and after SV40 transfection gene-immobilized liver cells. SV40 transfection gene-immobilized liver cells are able to synthesize glycogen and metabolize glucose.