Silencing effect of CD147 lentiviral vector on monocytes and macrophagocytes in peripheral blood of rabbits

Objective To study the expression of matrix metalloproteinase inducer(CD147) and screen the lentivirus vectors that highly suppress the CD147-expressed shRNA in monocytes and macrophagocytes in peripheral blood of rabbits. Methods Lentivirus vectors were constructed according to the sequence of CD147 mRNA from rabbits.The animals were divided into blank control group,negative control(NC) group,and 4 CD147 shRNA lentivirus groups(groups A-D).Seventy-two hours after monocytes and macrophagocytes were transfected into shRNA,the transfection results were observed by fluorescence microscopy.The expression of CD147 mRNA and protein was assayed by fluorescence quantitative RT-PCR and ELISA,respectively. Results RT-PCR showed that the expression level of CD147 mRNA and protein was(0.98±0.09)pg/ml and(119.69±16.40)pg/ml,(1.00±0.05)pg/ml and(115.66±13.88)pg/ml,(0.42±0.03)pg/ml and(58.74±5.11)pg/ml,(0.56±0.04)pg/ml and(70.99±7.42)pg/ml,(0.63±0.08)pg/ml and(82.79±7.71)pg/ml,(0.53±0.04)pg/ml and(69.71±5.84)pg/ml,respectively,in control group,NC group,and 4 shRNA lentivirus vector groups,72h after transfection.The CD147 shRNA lentivirus vectors inhibited the expression of CD147 mRNA and protein in macrophagocytes of about 57.72% and 50.92%(P<0.05). Conclusion The successfully designed and synthesized CD147 shRNA lentivirus vectors can specifically and effectively block the CD147-expressed shRNA.