Construction and identification of RNA interference lentivirus vector of Cav2.2 e37a gene

Objective To construct the RNA interference（RNAi） lentivirus vector of Cav2.2 e37a gene in order to inhibit the expression of Cav2.2 e37a gene. Methods An interfere plasmid vector, pLL3.7-Cav2.2e37a, was constructed and confirmed by sequencing according to the effective sequence of siRNA targeting Cav2.2 e37a gene identified in our previous study. 293 T cells were co-transfected with pRsv-REV, pMDlg-pRRE, pMD2G, and pLL3.7-Cav2.2e37a. Concentrated virus titer was detected by realtime PCR. Results The RNAi vector of Cav2.2 e37a gene（pLL3.7-Cav2.2e37a） producing Cav2.2 e37a shRNA was constructed. The concentrated titer of virus suspension was 1×10⁹Tu/ml. Conclusion RNAi lentivirus vector of Cav2.2 e37a gene can be constructed.