Construction of gastric cancer related gene GCRG213 eukaryotic expression vector and it’s effect on gastric cancer cells

Objctive: To investigate the effect of gene GCRG213 transfection（sense, antisense） on gastric cancer cell MKN45. Methods: Expression of GCRG213 was detected with semi-quantitative RT-PCR and Western Blot. The growth graph was protracted by the methods of cell counting. The clone formation rate in plate and in nude mice was calculated by the oncogenesis characters of transfected cells. Results: In vitro, the average clone formation rate increased in the cells transfected with pcDNA3.1-（sense） compared with those transfected with vector, while the average clone formation rate decreased in the cells transfected with pcDNA3.1-（antisense）. The time of oncogenesis of pcDNA3.1-（sense） transducted cells in nude mice was shorter and the tumor nodes were bigger. The time of oncogenesis of pcDNA3.1-（antisense） transducted cells in nude mice was prolonged and the tumor nodes were smaller.Conclusion: GCRG213 promotes tumor cell growth and induced the cells′ malignancy in vitro and vivo.