Expression of Nav1.8 in rat dorsal root ganglion neurons under different culture conditions

Objective: To investigate the expression of Nav1.8 in rat dorsal root ganglion（DRG） neurons under different（culture） conditions. Methods: By use of RT-PCR we observed the role of different blood serum and exogenous nerve growth factor（NGF） on the expression of Nav1.8 in rat DRG neurons. Results: NGF treatment can significantly upregulate the expression of Nav1.8 in the FBS+NGF group and HS+NGF group compared with the FBS and HS groups,while the Nav1.8 mRNA level showed no significant deference between FBS and HS groups. Conclusion: Exogenous NGF can upregulate the expression of（Nav1.8） in cultured rat DRG neuron,while serum of FBS or HS do not affect the growth of the neuron and the expression of Nav1.8.