Effect of oxidized low density lipoprotein on placental growth factor-1 influencing releasing of inflammatory cytokines in human umbilical vein endothelial cells

Objective To study the effect of oxidized low density lipoprotein（LDL）on placental growth factor-1（PGF-1）influencing releasing of inflammatory cytokines in human umbilical vein endothelial cells（ECV-304）. Methods Under the condition of oxidized LDL,ECV-304 cells（2×105）were incubated in RPMI-1640 medium containing 2%fetal bovine serum in the absence or presence of PGF-1（20,40,and 80ng/mL）for 3,6,12 and 24h,respectively.IL-6 and MMP-2 levels in the supernatant were measured using an ELISA quality kit. Results PGF-1 induced the secretion of inflammatory cytokines in a dose-and time-dependent manner.The expression level of IL-6 and MMP-2 was higher in ECV-304 cells under the condition than not under the condition of oxidized LDL. Conclusion Oxidizing injury is an independent risk factor for artherosclerosis.PGF can up-regulate the expression of inflammatory cytokines,and further deteriorate the disease.