A study of dehydrated alcohol intestinal mucosa treatment

Objective: To observe the results of the dehydrated alcohol intestinal mucosa treatment in rat for a long period. Methods: Using dehydrated alcohol soaking 0.5 min,1 min,or dehydrated alcohol applying 0.5 min,1 min,to treat the rat small intestinal mucosa.To observe the histological changes of the intestinal segments in rat bladder augmentation model,and the function changes of the mucosa in rat bladder substitution model. Results: 5 month postoperation,obvious fibrosis and different degree of contraction happened in the intestinal segment after treated by dehydrated alcohol soaking 1 min,0.5 min or by dehydrated alcohol applying 1 min;after treated by dehydrated alcohol applying 0.5 min,there is no shrink and no obvious fibrosis in the intestinal segments.1 month postoperation,treated by dehydrated alcohol applying 0.5 min,the weight of the residual mucus in the neobladder decreased significantly（P＜0.01）,from 2 months to 5 months postoperation,the weight of the residual mucus in the neobladder is similar to the control group（P＞0.05）.1 month and 5 month postoperation,treated by dehydrated alcohol applying 0.5 min,the quantity of water and electrolyte secreted or absorbed by the neobladder mucosa,compared with control group,the difference is not significant（P＞0.05）. Conclusion: Dehydrated alcohol has strong tissu infiltration,can easily injure the deep muscular layer of the rat intestinal segments when the mucosa treatment is performed,if the treatment time ＞（0.5 min）,that will lead to fibrosis in the intestinal segments at last;suitable treatment by dehydrated alcohol applying 0.5 min that not injure the muscular layer can only decrease the quantity of the mucus for a short piece of time in early postoperation,can not change the intestinal mucosa functions for a long period.